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Jumbo frames, changing MTU size without powering down guest VM

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I am trying to disable jumbo frames, specifically change the mtu size to 1500 (down from 9000) in vcenter on a few hosts. Some hosts are running esxi 4.1, others are running esxi 5.0. I am wondering if it is absolutely necessary to power down VMs that are connected to the iscsi san while making this change, or is it relatively safe to make this change while the VMs are running.

 

Each VM has multipathing enabled, 2 vswitches with 1 nic associated with each.

 

I know how to make the changes, my questions is regarding if it is possible to safely make the changes while VMs are still accessing the SAN.


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